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| Athlete | School | Time | Meet | Location | Date | ||||
| Qualified for Cities | |||||||||
| Provisional Qualification | |||||||||
| 300m | |||||||||
| 1 | Alison | Doway | Fr | Port Richmond | 43.54 | fat | Staten Island County Champs | Armory | 1/17/2010 |
| 2 | Karim | Brooks | Sr | Port Richmond | 43.98 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 3 | Breanna | Brukalo | So | MSIT | 44.73 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 4 | Brigitte | Pewu | Fr | Curtis | 46.22 | fat | Staten Island County Champs | Armory | 1/17/2010 |
| 5 | Meredith | Hansalik | Fr | MSIT | 46.26 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 6 | Brittany | Sheeran | Sr | Tottenville | 46.42 | fat | Staten Island County Champs | Armory | 1/17/2010 |
| 7 | Temitope | Omoladun | Sr | Tottenville | 46.43 | fat | Staten Island County Champs | Armory | 1/17/2010 |
| 8 | Tiara | Summers | Jr | Port Richmond | 46.68 | fat | Rhody Classic | Kingston, RI | 1/9/2010 |
| 9 | Nynneya | Columbus | Jr | Port Richmond | 46.71 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 10 | Deanna | Veasey | So | MSIT | 46.90 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 11 | Amanda | Feldstein | Jr | Petrides | 47.46 | fat | Jim McKay Team Challenge | Armory | 12/5/2009 |
| 12 | Laura | Soffer | Sr | MSIT | 47.52 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 13 | Corinne | Birchard | Fr | Curtis | 47.84 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 14 | Shi Anne | Humes | Fr | Port Richmond | 47.93 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 15 | Nicole | Tantillo | Fr | Tottenville | 48.08 | fat | Jim McKay Games | Armory | 12/6/2009 |
| 16 | Tanisha | Ross | Jr | Port Richmond | 48.13 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 17 | Terren | Huggins | Sr | Port Richmond | 48.16 | fat | Bishop Loughlin Games | Armory | 12/19/2009 |
| 18 | Natalia | Krawiec | Jr | Petrides | 48.62 | fat | Staten Island County Champs | Armory | 1/17/2010 |
| 19 | Mary Rose | Konuwa | Fr | Curtis | 48.90 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 20 | Liane | Conte | Sr | MSIT | 48.93 | fat | Staten Island County Champs | Armory | 1/17/2010 |
| 21 | Stephanie | Candela | So | Susan Wagner | 49.02 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 22 | Robyn | Schiff | Jr | MSIT | 49.12 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 23 | Kassandra | Somers | So | Port Richmond | 49.31 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 24 | Katiana | Hill | Fr | Port Richmond | 49.39 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 25 | Danielle | Sorrentino | Jr | Port Richmond | 49.51 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 25 | Brittani | Young | Jr | Port Richmond | 49.51 | fat | A Night at the Sprints | Armory | 12/30/2009 |
