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| School | Time | Meet | Location | Date | ||
| Qualified for Cities | ||||||
| Provisional Qualification | ||||||
| 4 x 200m | ||||||
| 1 | Benjamin Cardozo | 1:41.90 | fat | Martin L. King Jr. Relays | Armory | 1/18/2010 |
| 2 | Benjamin Cardozo ’B’ | 1:47.25 | fat | Martin L. King Jr. Relays | Armory | 1/18/2010 |
| 3 | Campus Magnet | 1:48.89 | fat | Martin L. King Jr. Relays | Armory | 1/18/2010 |
| 4 | Jamaica | 1:50.43 | fat | PSAL Holiday Games | Armory | 12/26/2009 |
| 5 | Benjamin Cardozo ’C’ | 1:52.26 | fat | Martin L. King Jr. Relays | Armory | 1/18/2010 |
| 6 | Springfield Gardens | 1:56.62 | fat | Bishop Loughlin Games | Armory | 12/19/2009 |
| 7 | John Bowne | 1:57.60 | fat | Martin L. King Jr. Relays | Armory | 1/18/2010 |
| 8 | Martin Van Buren | 1:58.60 | fat | Pilgrim Games | Armory | 11/29/2009 |
| 9 | Hillcrest | 1:59.94 | fat | Bishop Loughlin Games | Armory | 12/19/2009 |
| 10 | Thomas Edison | 2:00.47 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 11 | Townsend Harris | 2:00.71 | fat | PSAL Holiday Games | Armory | 12/26/2009 |
| 12 | John Adams | 2:00.79 | fat | Pilgrim Games | Armory | 11/29/2009 |
| 13 | Grover Cleveland | 2:01.62 | fat | Hispanic Games | Armory | 1/9/2010 |
| 14 | Bayside | 2:01.74 | fat | Pilgrim Games | Armory | 11/29/2009 |
| 15 | Young Womens Leadership | 2:02.32 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 16 | Jamaica ’B’ | 2:02.51 | fat | PSAL Holiday Games | Armory | 12/26/2009 |
| 17 | Flushing | 2:04.13 | fat | Bishop Loughlin Games | Armory | 12/19/2009 |
| 18 | Campus Magnet ’B’ | 2:04.74 | fat | Martin L. King Jr. Relays | Armory | 1/18/2010 |
| 19 | Queens Teaching | 2:06.67 | fat | Martin L. King Jr. Relays | Armory | 1/18/2010 |
| 20 | Richmond Hill | 2:08.35 | fat | Martin L. King Jr. Relays | Armory | 1/18/2010 |
| 21 | Francis Lewis | 2:09.27 | fat | Pilgrim Games | Armory | 11/29/2009 |
| 22 | Martin Van Buren ’B’ | 2:09.82 | fat | Martin L. King Jr. Relays | Armory | 1/18/2010 |
| 23 | John Adams ’B’ | 2:11.41 | fat | PSAL Holiday Games | Armory | 12/26/2009 |
| 24 | John Bowne ’B’ | 2:11.59 | fat | Pilgrim Games | Armory | 11/29/2009 |
| 25 | Forest Hills | 2:11.68 | fat | New Balance Games | Armory | 1/23/2010 |
