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| School | Time | Meet | Location | Date | ||
| Qualified for Cities | ||||||
| Provisional Qualification | ||||||
| SHR (4 x 55hh) | ||||||
| 1 | Sheepshead Bay | 36.80 | fat | New Balance Games | Armory | 1/23/2010 |
| 2 | Clara Barton | 39.55 | fat | New Balance Games | Armory | 1/23/2010 |
| SHR (3 x 55hh) | ||||||
| 1 | Clara Barton | 30.25 | fat | Martin L. King Jr. Relays | Armory | 1/18/2010 |
