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| Athlete | School | Time | Meet | Location | Date | ||||
| Qualified for Cities | |||||||||
| Provisional Qualification | |||||||||
| 300m | |||||||||
| 1 | John | Thomas | Sr | Sheepshead Bay | 33.95 | fat | Bishop Loughlin Games | Armory | 12/19/2009 |
| 2 | Andre | Jordan | Jr | Medgar Evers | 34.34 | fat | PSAL Mid Winter Classic | Armory | 1/30/2010 |
| 3 | Reginald | Mortel | Sr | Clara Barton | 35.45 | fat | PSAL Holiday Games | Armory | 12/26/2009 |
| 4 | Khamani | Davis Parker | Sr | Boys & Girls | 35.80 | fat | PSAL Mid Winter Classic | Armory | 1/30/2010 |
| 5 | Adrian | Lewis | Jr | Medgar Evers | 35.84 | fat | PSAL Mid Winter Classic | Armory | 1/30/2010 |
| 6 | Donald | Williams Jr | Sr | Midwood | 35.88 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 7 | Fard | Rollock | Sr | Transit Tech | 35.94 | fat | PSAL Mid Winter Classic | Armory | 1/30/2010 |
| 8 | Andy | Nicolas | So | Midwood | 36.02 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 9 | Rohan | Stewart | Sr | Boys & Girls | 36.04 | fat | PSAL Mid Winter Classic | Armory | 1/30/2010 |
| 10 | Daniel | Simon | Jr | Boys & Girls | 36.24 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 11 | Javaun | Grant | So | Boys & Girls | 36.64 | fat | PSAL Mid Winter Classic | Armory | 1/30/2010 |
| 12 | Matthew | West | Sr | Medgar Evers | 36.74 | fat | PSAL Mid Winter Classic | Armory | 1/30/2010 |
| 13 | Kajeem | Bailey | Jr | Transit Tech | 36.76 | fat | Bishop Loughlin Games | Armory | 12/19/2009 |
| 14 | Adrain | Palmer | Jr | Clara Barton | 36.87 | fat | Bishop Loughlin Games | Armory | 12/19/2009 |
| 15 | Charles | Russell | Sr | Sheepshead Bay | 37.03 | fat | Bishop Loughlin Games | Armory | 12/19/2009 |
| 16 | Calvin | Edmonds | Sr | James Madison | 37.05 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 17 | Akeam | Allen | Sr | Sheepshead Bay | 37.08 | fat | Bishop Loughlin Games | Armory | 12/19/2009 |
| 18 | Dane | Distin | Jr | Abraham Lincoln | 37.15 | fat | Jim McKay Team Challenge | Armory | 12/5/2009 |
| 19 | Kyle | Patrice | Jr | Medgar Evers | 37.20 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 20 | John | Crawford | Sr | William E. Grady | 37.37 | fat | Bishop Loughlin Games | Armory | 12/19/2009 |
| 21 | Jason | Genius | Sr | Medgar Evers | 37.42 | fat | Jim McKay Games | Armory | 12/6/2009 |
| 22 | Clayton | Crooks | So | Samuel J. Tilden | 37.52 | fat | Bishop Loughlin Games | Armory | 12/19/2009 |
| 23 | Ryan | Douglas | Sr | Samuel J. Tilden | 37.53 | fat | Bishop Loughlin Games | Armory | 12/19/2009 |
| 24 | Lyndon | Samuel | Jr | Erasmus | 37.62 | fat | PSAL Holiday Games | Armory | 12/26/2009 |
| 25 | Denroy | Lawrence | Sr | Harry Van Arsdale | 37.64 | fat | Jim McKay Team Challenge | Armory | 12/5/2009 |
