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| Athlete | School | Time | Meet | Location | Date | ||||
| Qualified for Cities | |||||||||
| Provisional Qualification | |||||||||
| 300m | |||||||||
| 1 | Davion | Wint | Jr | Christopher Columbus | 34.79 | fat | PSAL Mid Winter Classic | Armory | 1/30/2010 |
| 2 | Hakeem | Sackes Bramble | Sr | Taft | 36.10 | fat | PSAL Mid Winter Classic | Armory | 1/30/2010 |
| 3 | Bertrand | Oubida | Sr | Taft | 36.79 | fat | PSAL Mid Winter Classic | Armory | 1/30/2010 |
| 4 | Godfrey | Asante | So | DeWitt Clinton | 37.09 | fat | Bishop Loughlin Games | Armory | 12/19/2009 |
| 5 | Strymar | Livingston | So | Christopher Columbus | 37.22 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 6 | Weyonn | Hoskins | Jr | Harry S. Truman | 37.44 | fat | Jim McKay Team Challenge | Armory | 12/5/2009 |
| 7 | Jeremiah | Obeng Agyapong | So | DeWitt Clinton | 37.73 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 8 | Carlos | Dipre | Sr | Christopher Columbus | 38.03 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 9 | Kemar | Newman | Sr | Taft | 38.12 | fat | Bishop Loughlin Games | Armory | 12/19/2009 |
| 10 | Christophe | Baker | Sr | Samuel Gompers | 38.22 | fat | PSAL Holiday Games | Armory | 12/26/2009 |
| 11 | Jason | Hewitt | Sr | Harry S. Truman | 38.32 | fat | Jim McKay Team Challenge | Armory | 12/5/2009 |
| 12 | Brandon | Oneil | Jr | Evander | 38.37 | fat | PSAL Holiday Games | Armory | 12/26/2009 |
| 13 | Jonathan | Lee | Sr | Bronx Science | 38.38 | fat | Stanner Games | Armory | 1/16/2010 |
| 14 | Aaron | Roseman | Jr | Bronx Science | 38.45 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 15 | Roberto | Cabral | Sr | John F. Kennedy | 38.53 | fat | PSAL Holiday Games | Armory | 12/26/2009 |
| 16 | Alex | Dankwa | So | DeWitt Clinton | 38.62 | fat | Jim McKay Team Challenge | Armory | 12/5/2009 |
| 17 | Warren | Asphall | So | Harry S. Truman | 38.72 | fat | Jim McKay Team Challenge | Armory | 12/5/2009 |
| 18 | Kemare | Johnson | Sr | Herbert H. Lehman | 38.87 | fat | Stanner Games | Armory | 1/16/2010 |
| 19 | Kernie | Williams | Jr | Samuel Gompers | 38.99 | fat | Jim McKay Team Challenge | Armory | 12/5/2009 |
| 20 | Romario | Palmer | So | Harry S. Truman | 39.00 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 21 | Arnoldo | Burnett | So | John F. Kennedy | 39.01 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 22 | Jason | Tomlinson | So | Harry S. Truman | 39.11 | fat | PSAL Holiday Games | Armory | 12/26/2009 |
| 23 | Wayne | Ferrance | So | John F. Kennedy | 39.24 | fat | A Night at the Sprints | Armory | 12/30/2009 |
| 24 | Rachard | Byers | Jr | DeWitt Clinton | 39.38 | fat | Jim McKay Team Challenge | Armory | 12/5/2009 |
| 25 | Denvor | Hawthorne | Jr | DeWitt Clinton | 39.40 | fat | A Night at the Sprints | Armory | 12/30/2009 |
